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目的 研究人肺癌A5 49细胞和乳腺癌MCF7细胞对市售 4种中药制剂 (斑蟊酸钠、苦参素、华蟾素和川芎素 )的敏感性 ,以及川芎素影响肺癌A5 49细胞增殖的可能机制。方法 采用MTT法测定药物对细胞增殖的抑制率和药物间的协同抑制作用 ;流式细胞法测定细胞周期。结果 4种中药对乳腺癌MCF7细胞的增殖均有不同程度的抑制作用 ,但只有川芎素可明显抑制肺癌A5 49细胞的增殖 ;川芎素与 3种化疗药物联合对A5 49细胞有协同作用 ;川芎素可诱导A5 49细胞凋亡。结论 斑蟊酸钠、苦参素和华蟾素对不同肿瘤细胞的抑制效果差异很大 ;川芎素不仅有抑制肿瘤细胞增殖的作用 ,还与化疗药物有协同效果 ;川芎素抑制A5 49细胞增殖的机制与其诱导细胞凋亡有关。
Objective To investigate the sensitivity of human lung cancer A549 cells and breast cancer MCF7 cells to four kinds of traditional Chinese medicines (sodium cantharidate, oxymatrine, cinobufacini and sodium ferulate) and the effects of sodium ferulate on the proliferation of lung cancer A549 cells Possible mechanism. Methods MTT assay was used to determine the inhibitory rate of drug on cell proliferation and synergistic inhibition between drugs. Flow cytometry was used to determine the cell cycle. Results Four kinds of Chinese herbs could inhibit the proliferation of breast cancer MCF7 cells to varying degrees, but only the four kinds of drugs could inhibit the proliferation of lung cancer A549 cells. The combination of sodium ferulate and three kinds of chemotherapy drugs had synergistic effect on A549 cells. Chuanxiong Su-induced A549 cell apoptosis. Conclusion Sodium cantharidin, matrine and cinobufacini have different effects on different tumor cells. Chuanxiongling not only has the effect of inhibiting tumor cell proliferation, but also has synergistic effect with chemotherapeutic drugs. The mechanism is related to its induction of apoptosis.