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目的:探讨非小细胞肺癌胸水细胞块在间充质上皮转化因子受体(c-mesenchymal-epithelial transition,c-M ET)基因扩增检测中的临床价值。方法:采用RT-PCR法检测215例非小细胞肺癌细胞块和404例非小细胞肺癌组织块中c-MET基因扩增,并检测细胞块同时送检组织块的患者74例的一致性。结果:细胞块c-MET基因扩增31例,扩增率14.42%(31/215);组织块c-MET基因扩增3 5例,扩增率8.6 6%(3 5/4 0 4);7 4例有组织块对照的细胞块c-M ET结果一致性有6 8例,一致率达9 1.8 9%(6 8/7 4),其中细胞块c-M ET基因扩增率1 2.1 6%(9/7 4),组织块扩增率1 7.5 7%(1 3/7 4)。结论:非小细胞肺癌胸水细胞块c-MET的扩增率略高于组织块;有恶性胸水的非小细胞肺癌患者原发灶组织发生c-MET扩增的概率较高。
Objective: To investigate the clinical value of detecting pleural effusion cells in non-small cell lung cancer (c-M ET) gene amplification in c-Mesenchymal-epithelial transition. Methods: The amplification of c-MET gene was detected by RT-PCR in 215 non-small cell lung cancer cell masses and 404 non-small cell lung cancer cell masses. Results: The c-MET gene amplification was performed in 31 cases with a rate of 14.42% (31/215). The c-MET gene amplification was performed in 35 cases with a rate of 8.66% (3 5/4 0 4) . The consistency of cM ET in 74 cases with tissue block was 68%, and the concordance rate was 9 1.8 9% (6 8/7 4). The amplification rate of cM ET gene in cell block was 11.6% ( 9/7 4). The rate of tissue block expansion was 7.57% (1 3/7 4). CONCLUSIONS: The c-MET amplification rate of pleural effusion cells in non-small cell lung cancer is slightly higher than that of the tissue mass. The probability of c-MET amplification in the primary tumor tissue of patients with malignant pleural effusion is higher.