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SSTA是一种多价同种异型抗血清,可在简易微量细胞毒技术中,用以快速检测RT1抗原、次要组织相容性抗原和其它细胞膜分化抗原。本文报道采用大鼠混合淋巴细胞多次腹腔免疫的方法,制备F_(344)、Lou、SHR、WKy4系大鼠的SSTA,并用以对比监测了10个非相关品系大鼠及其F_1。结果表明;本品SSTA对同源大鼠品系淋巴细胞毒试验阴性,对其它品系和F_1大鼠反应阳性,可作为该4个近交系大鼠的定型血清。文中还对RT1抗原产生的淋巴细胞毒性作用以及在移植物排斥中的作用进行了探讨。
SSTA is a multivalent allogeneic antiserum that can be used for rapid detection of RT1 antigens, secondary histocompatibility antigens and other cell membrane antigen in a simple micro-cytotoxicity assay. This paper reports the preparation of SSTA in F_ (344), Lou, SHR and WKy4 rats by multiple intraperitoneal immunization of mixed lymphocytes in rats, and compared and monitored F_1 in 10 non-related strains of rats. The results showed that SSTA of this product was negative to lymphocyte cytotoxicity test in homologous rat strains and positive to other strains and F_1 rats, which could be used as the typing serum of 4 inbred rats. The article also discussed the role of RT1 antigen in lymphocyte toxicity and graft rejection.