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目的探讨多乐氟联合窝沟封闭剂治疗后对儿童唾液内的内氏放线菌(Actinomyces naeslundii,An)和龋齿放线菌(Actinomyces odontolyticus,Ao)的影响。方法收集45例多乐氟联合窝沟封闭剂和35例单纯行窝沟封闭剂治疗前后的龋病患儿唾液,提取细菌总DNA,实时荧光定量PCR检测唾液总菌、An和Ao含量。结果高龋、中龋患儿唾液An、Ao数量均显著高于无龋患儿(P<0.05)。高龋患儿和中龋患儿之间唾液An、Ao数量及百分含量差异均无统计学意义(P>0.05)。多乐氟联合窝沟封闭剂治疗后,龋病患儿唾液An和Ao含量显著降低(P<0.05),且比单纯窝沟封闭剂治疗后下降更为明显,差异有统计学意义(P<0.05)。结论多乐氟联合窝沟封闭剂对龋病的保护作用可能与抑制口腔An和Ao生长有关。
Objective To investigate the effects of doxorubicin combined with pit and fissure sealant on Actinomyces naeslundii (An) and Actinomyces odontolyticus (Ao) in saliva of children. Methods Salivary samples from 45 children with caries were collected before and after treatment with 45 cases of dl-fluoro-fissure sealant and 35 cases of simple pit-fissure sealant. The total bacterial DNA was extracted and the contents of An and Ao in saliva were detected by real-time fluorescence quantitative PCR. Results The numbers of saliva An, Ao in children with caries were significantly higher than those without caries (P <0.05). There was no significant difference in the numbers and percentage of salivary An, Ao between children with caries and those with caries (P> 0.05). The levels of An and Ao in saliva of children with caries decreased significantly (P <0.05) after treatment with doxorubicin combined with pit and fissure sealant, and the difference was statistically significant (P < 0.05). Conclusions The protective effect of doxorubicin combined with pit and fissure sealant on dental caries may be related to the inhibition of oral An and Ao growth.