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目的:通过检测a-L-岩藻糖苷酶(AFU)在甲胎蛋白(AFP)阳性相关疾病及原发性肝癌(PHC)中的活性水平并与AFP进行比较,评价AFU对PHC的诊断价值。方法:采用显色多肽基质检测法检测AFU的活性水平;采用放免法测定AFP。结果:PHC患者血清AFU、AFP的阳性率明显高于其他各疾病组,与其他各疾病组比较P<0.05。PHC组AFP、AFU阳性率分别为74.6%、76.0%,两者比较差异无显著性(P>0.05),与AFP诊断PHC的敏感性和特异性相似。AFU最大的特点是其与血AFP值、肿瘤的大小无关,其在AFP阴性的PHC中阳性率高,在肿块<3cm的PHC中阳性率高。结论:AFU在诊断PHC方面确实具有较好的敏感性和特异性。
OBJECTIVE: To evaluate the diagnostic value of AFU for PHC by detecting the activity of a-L-fucosidase (AFU) in AFP-positive related diseases and primary liver cancer (PHC) and comparing with AFP. Methods: The activity of AFU was detected by colorimetric peptide matrix assay. AFP was determined by radioimmunoassay. Results: The positive rates of serum AFU and AFP in PHC patients were significantly higher than those in other disease groups, P <0.05 compared with other disease groups. The positive rates of AFP and AFU in PHC group were 74.6% and 76.0% respectively, with no significant difference (P> 0.05), which was similar to the sensitivity and specificity of AFP in diagnosing PHC. The most prominent feature of AFU is its blood AFP value, tumor size has nothing to do, its positive rate in AFP negative PHC high, in the mass <3cm PHC positive rate. Conclusion: AFU does have a good sensitivity and specificity in the diagnosis of PHC.