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目的对ｒＩＬ－１、ｒＴＮＦα、ＬＰＳ、ｒＩＬ－１ｒａ等刺激因子调节人脐静脉内皮细胞（ＨＵＶＥＣ）表达分泌ＩＬ－６及ＩＬ－８进行研究，探求血管内皮细胞（ＥＣ）在炎症发生中的作用，观察ｒＩＬ－１ｒａ对炎性反应的抑制效应。方法ＨＵＶＥＣ原代培养，ＨＵＶＥＣ释放ＩＬ－６、ＩＬ－８观察，ＭＴＴ比色法作ＩＬ－６活性测定，双抗夹心ＥＬＩＳＡ法测ＩＬ－８，ｔ检验进行统计学处理。结果未刺激的ＨＵＶＥＣ培养２４小时上清ＩＬ－６活性为１８１±２２Ｕ／ｍｌ。增加ＬＰＳ、ｒＩＬ－１及ｒＴＮＦα的诱导浓度或延长诱导时间，ＩＬ－６释放量也随之增加。刺激因子诱导后的ＨＵＶＥＣ培养上清ＩＬ－８浓度与刺激因子浓度呈正相关。动力学显示，刺激１小时的ＨＵＶＥＣ培养上清即可检测到ＩＬ－８升高，２４小时达峰值。ｒＩＬ－１ｒａ对ｒＩＬ－１诱导的ＨＵＶＥＣ释放ＩＬ－６和ＩＬ－８均表现特异的抑制作用，当ｒＩＬ－１ｒａ量达１０ｎｇ（相对于１ＵＩＬ－１）时，ＩＬ－６、ＩＬ－８的产生明显受抑。ＬＰＳ诱导的ＨＵＶＥＣ释放ＩＬ－６、ＩＬ－８则不能被ｒＩＬ－１ｒａ抑制。结论ｒＩＬ－１ｒａ具有抗炎症效应，有望作为一种新的抗炎药物应用于临床，但因其有效剂量用量大，目前普及?
Objective To investigate the effects of rIL-1, rTNFα, LPS and rIL-1ra on the expression of IL-6 and IL-8 in human umbilical vein endothelial cells (HUVECs) and explore the role of vascular endothelial cells (ECs) , To observe the inhibitory effect of rIL-1ra on the inflammatory response. Methods HUVEC primary culture, HUVEC release of IL-6, IL-8 observation, MTT colorimetric determination of IL-6 activity, double antibody sandwich ELISA assay IL-8, t test for statistical analysis. Results The supernatant IL-6 activity of unstimulated HUVEC cultured for 24 hours was 181 ± 22 U / ml. Increase of LPS, rIL-1 and rTNFα induced concentration or prolonged induction time, IL-6 release also increased. The concentration of IL-8 in supernatant of HUVEC induced by stimulus was positively correlated with the concentration of stimulus. Kinetics showed elevated IL-8 levels were detected in HUVEC culture supernatants stimulated for 1 hour and peaked at 24 hours. rIL-1ra showed specific inhibitory effect on the release of IL-6 and IL-8 by HUVECs induced by rIL-1. The production of IL-6 and IL-8 was detected when the amount of rIL-1ra was 10ng (relative to 1UIL-1) Obviously suppressed. IL-6 was released from LPS-induced HUVECs and IL-8 was not inhibited by rIL-1ra. Conclusion rIL-1ra has an anti-inflammatory effect and is expected to be used as a new anti-inflammatory drug in clinical practice. However, rIL-1ra is widely used because of its effective dosage and dosage.