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目的探讨LIN28B基因rs314277和rs314276位点在宁夏地区回、汉族群体中及不同性别间的分布特征。方法研究对象来自宁夏医科大学2011级健康学生共705例,其中男性回、汉族分别为144例和167例,女性回、汉族分别为188例和206例。SNPs分型均采用基因测序来检测。结果宁夏回族群体LIN28B基因两个多态位点基因型频率及等位基因频率分别为:男性rs314277(AC:4.5%,CC:38.9%;A:2.3%,C:41.1%);rs314276(AA:2.7%,AC:21.4%,CC:19.3%;A:13.4%,C:30.0%);女性rs314277(AA:0.6%,AC:7.5%,CC:48.5%;A:4.4%,C:52.3%);rs314276(AA:3.3%,AC:28.9%,CC:24.4%;A:17.8%,C:38.9%);宁夏汉族群体LIN28B基因两个多态位点基因型频率及等位基因频率分别为:男性rs314277(AC:3.2%,CC:41.6%;A:1.6%,C:43.2%);rs314276(AA:4.6%,AC:17.4%,CC:22.8%;A:13.3%,C:31.5%);女性rs314277(AC:4.6%,CC:50.7%;A:2.3%,C:52.9%);rs314276(AA:4.8%,AC:18.8%,CC:31.6%;A:14.2%,C:41.0%)。结论宁夏人群LIN28B基因rs314277多态性位点等位基因频率在回、汉族之间差异有显著性(P<0.05);rs314276多态性位点基因型频率在回、汉族之间差异有显著性(P<0.05);LIN28B基因两个多态性位点在宁夏不同性别之间差异无显著性(P>0.05);宁夏人群两个多态性位点基因型和等位基因型频率的分布与人类基因组计划扫描所得不同种族人群相比差异有显著性(P<0.05)。
Objective To investigate the distribution of rs284227 and rs314276 loci in Linxia region, Han population and different genders in Ningxia area. METHODS: A total of 705 health-seeking students from Ningxia Medical University were enrolled in this study. Among them, 144 were male and 167 were Han Chinese, while 188 were Han and 206 were Han Chinese. SNPs typing using gene sequencing to detect. Results The frequencies of genotypes and alleles of LIN28B gene in Ningxia Hui population in Ningxia were male rs314277 (AC: 4.5%, CC: 38.9%, A: 2.3%, C: 41.1%), rs314276 : AC: 21.4%, CC: 19.3%; A: 13.4%, C: 30.0%); women rs314277 (AA: 52.3%); rs314276 (AA: 3.3%, AC: 28.9%, CC: 24.4%; A: 17.8%, C: 38.9%); genotype frequencies and alleles of two polymorphic loci of LIN28B gene in Ningxia Han population The frequencies of rs314277 (AC: 3.2%, CC: 41.6%; A: 1.6%, C: 43.2%); rs314276 (AA: 4.6%; AC: 17.4%; C: 31.5%); women rs314277 (AC: 4.6%; CC: 50.7%; A: 2.3%; C: 52.9%); rs314276 (AA: 4.8%; AC: 18.8%; %, C: 41.0%). Conclusion The frequency of rs314277 polymorphism loci in Ningxia population was significantly different between Hui and Han (P <0.05). The genotype frequency of rs314276 locus was significantly different between Hui and Han nationality (P <0.05). There was no significant difference in the genotype frequencies and allele frequencies between the two genotypes of LIN28B gene in different genders in Ningxia (P> 0.05) There were significant differences (P <0.05) compared with those from different ethnic groups screened by human genome project.