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种子储藏蛋白质主要由谷蛋白、醇溶谷蛋白和球蛋白组成。这些蛋白质在种子发育期被合成后,经过区室化过程,被蓄积在胚乳中。本研究系统分析了水稻57H突变体的表型多样性,旨在为胚乳储藏蛋白质的遗传调节机制的全面解明展示一个新的前景。胚乳蛋白质的SDS-PAGE分析和免疫印迹分析显示了高量含有57kD谷蛋白前体的水稻57H突变体系列具有多样的储藏蛋白质表型。基于其表型的多样性,57H突变体系列被分成了三种类型。与野生型水稻品种相比,所有的类型Ⅰ突变体(glup4,glup6,Glup5,esp2)不仅高量蓄积谷蛋白前体、少量蓄积成熟型谷蛋白的40kD酸性亚基和20kD碱性亚基,而且显著减少了13kD醇溶谷蛋白b组分和26kDa球蛋白的蓄积;类型Ⅱ突变体(Glup1,glup2)不仅高量蓄积谷蛋白前体,还减少了26kDa球蛋白的蓄积;类型Ⅲ突变体(glup3)除了高量蓄积谷蛋白前体、少量蓄积成熟型谷蛋白亚基之外,并没有减少其它储藏蛋白质的蓄积。结果指出了57H变异系列对储藏蛋白的蓄积具有多样的影响,实质上是关于储藏蛋白质区室化的遗传体系。并就该遗传体系对储藏蛋白质的翻译后区室化及其营养性状的可能的影响进行了讨论。
Seed storage proteins are mainly composed of gluten, prolamin and globulin. After these proteins are synthesized during seed development, they are accumulated in the endosperm through a compartmentalization process. This study systematically analyzed the phenotypic diversity of rice 57H mutants and aims to provide a new perspective for a complete elucidation of the genetic regulatory mechanism of endosperm storage proteins. SDS-PAGE analysis and immunoblot analysis of endosperm proteins showed that a high number of rice 57H mutant lines containing 57 kD glutelin precursors have diverse storage protein phenotypes. Based on its phenotypic diversity, the 57H mutant series is divided into three types. All type I mutants (glup4, glup6, Glup5, esp2) not only accumulated high amounts of gluten precursors, but also accumulated 40 kD acidic subunits and 20 kD basic subunits of mature gluten in small quantities compared to wild type rice cultivars, And significantly reduced the accumulation of 13 kD prolamin b component and 26 kDa globulin; Type II mutants (Glup1, glup2) not only accumulated high amount of gluten precursors but also reduced 26 kDa globulin; Type III mutants (glup3) did not reduce the accumulation of other storage proteins except for the high accumulation of gluten precursors and the accumulation of a few mature gluten subunits. The results indicate that the 57H mutation series have diverse effects on the accumulation of storage proteins and are essentially genetic systems that compartmentalize storage proteins. The possible impact of this genetic system on the post-translational compartmentalization of storage proteins and their nutritional traits was also discussed.