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目的分析真菌诱导的白桦悬浮体系中N、P的吸收利用和三萜合成的关系。方法在白桦悬浮细胞的生长末期添加40μg/mL真菌诱导子,采用比色法分析真菌诱导后白桦悬浮体系中N、P和三萜量的变化。结果白桦细胞的干质量、三萜量和产量随着真菌处理时间的延长表现为逐渐降低趋势。其中,三萜量和产量均于真菌诱导后第1天达到最高,分别为21.98 mg/g和123.82 g/L,约为对照的2倍;白桦悬浮体系的pH值和电导率在真菌诱导后第1天达到高峰,pH值增长了6.10%,电导率增加了8.20%;除细胞内磷酸根质量分数在诱导的第1、2天分别降低了28.67%、15.68%外,真菌诱导使白桦悬浮培养体系中硝酸根、铵根、磷酸根量基本呈增加趋势。进一步相关性分析发现,真菌诱导使胞外磷酸根、铵根和硝酸根质量分数与三萜量的相关性显著提高。结论真菌诱导后白桦三萜合成的增加可能与磷酸根、硝酸根和铵根质量分数的变化相关。
Objective To analyze the relationship between absorption and utilization of N, P and triterpene synthesis in fungal-induced birch suspension. Methods 40μg / mL fungal elicitors were added to the suspension of birch suspension at the end of growth stage, and the contents of N, P and triterpene in the suspension of birch were measured by colorimetry. Results The dry weight, triterpene production and yield of Betula platyphylla decreased gradually with the prolongation of fungal treatment time. Among them, the amount and yield of triterpene were the highest on the first day after fungal induction, which were 21.98 mg / g and 123.82 g / L, respectively, about twice of the control. The pH value and conductivity of the suspension were The first day reached the peak, the pH value increased by 6.10% and the conductivity increased by 8.20%. In addition to the intracellular phosphate content decreased by 28.67% and 15.68% on the first and second day of induction, respectively, The amount of nitrate, ammonium and phosphate in the cultivation system basically increased. Further correlation analysis found that fungi induced extracellular phosphate, ammonium and nitrate mass fraction and triterpenoid correlation was significantly increased. Conclusion The increase of triterpene synthesis after fungal induction may be related to the change of mass fraction of phosphate, nitrate and ammonium.