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弓形虫感染由母体向胎儿传播,在人和 兽医中都属重要,迄今,包括小鼠在内的多种动物都已成功地用无毒、非持续性的弓形虫Ts-4温敏变异体(以下简称Ts-4)活速殖子作疫苗接种而获得抗攻击感染的成功。为进一步观察Ts-4对成、幼鼠的感染特性以及经胎盘、乳汁传播情况,作者用小鼠模型进行3组实验。弓形虫Ts-4速殖子系从接种在人包皮成纤维细胞(Hs68),在32.5℃,5％CO_2孵育后收集纯化。所用的小鼠为商品化的远交系HSD:ICR株雌性小鼠。
Toxoplasma infection is transmitted from the mother to the fetus and is important in both humans and veterinarians. To date, many animals, including mice, have been successfully treated with the non-toxic, non-persistent T. gondii Ts-4 temperature-sensitive mutant (Hereinafter referred to as Ts-4) tachyzoites for vaccination and get the success of anti-attack infection. In order to further observe the infection characteristics of Ts-4 into pups and young rats and the transmission through the placenta and milk, the authors conducted three experiments in a mouse model. Toxoplasma Ts-4 tachyzoites were collected and purified from human foreskin fibroblasts (Hs68) and incubated at 32.5 ° C, 5% CO 2. Mice used were commercial outbred HSD: ICR strain female mice.