Azospirillum brasilense and Pseudomonas fluorescens are well-known plant growth promoting rhizobacteria. However, the effects of A. brasilense and P. fluorescens on the N cycles in the paddy field and rice plant growth are little known. This study investigated whether and how A. brasilense and P. fluorescens contribute to the N transformations and N supply capacities in the rhizosphere, and clarified the effects of A. brasilense and P. fluorescens on the N application rate in rice cultivation. Inoculations with A. brasilense and P. fluorescens coupled with N application rate trials were conducted in the paddy field in 2016 and 2017. The inoculations of rice seedlings included four treatments: sterile saline solution (M0), A. brasilense (Mb), P. fluorescens (Mp), and co-inoculation with a mixture of A. brasilense and P. fluorescens (Mbp). The N application rate included four levels: 0 kg N ha–1 (N0), 90 kg N ha–1 (N90), 180 kg N ha–1 (N180), and 270 kg N ha–1 (N270). The results indicated that the Mbp and Mp treatments significantly enhanced the ammonification activities in the rhizosphere compared with the M0 treatment, especially for higher N applications, while the Mbp and Mb treatments greatly enhanced the nitrogenase activities in the rhizosphere compared with the M0 treatments, especially for lower N applications. Azospirillum brasilense and P. fluorescens did not participate in the nitrification processes or the denitrification processes in the soil. The soil respiration rate and microbial biomass N were greatly affected by the interactions between the rhizobacteria inoculations and the N fertilizer applications. In the Mbp treatment, N supply capacities and rice grain yields showed no significant differences among the N90, N180, and N270 applications. The N application rate in the study region can be reduced to 90 kg N ha–1 for rice seedlings co-inoculated with a mixture of A. brasilense and P. fluorescens.