论文部分内容阅读
在传代培养的ＨｅＬａ细胞上观察了叶酸对脂质体靶向摄取功能的影响。将内含钙黄绿素的叶酸．脂质体和脂质体分别加入ＨｅＬａ细胞中培养，并以荧光法检测细胞摄取量。共同培养４ｈ，细胞摄取叶酸一脂质体量为脂质体４倍以上，在０．４５ｍｇ· ｍＬ~（－１）时达饱和；过量游离叶酸竞争抑制细胞对叶酸－脂质体的摄取；磷脂酶Ｄ或磷脂酰肌醇特异性磷脂酶Ｃ通过影响叶酸受体而抑制细胞对叶酸－脂质体的摄取。因此，ＨｅＬａ细胞主要通过叶酸受体途径介导摄取叶酸－脂质体：叶酸受体高表达或高活性细胞摄取叶酸－脂质体的能力较未接叶酸的脂质体显著提高。
The effects of folic acid on the targeted uptake of liposomes were observed on subcultured HeLa cells. Will contain calcein folic acid. Liposomes and liposomes were added into HeLa cells respectively and the cell uptake was measured by fluorescence method. After co-cultured for 4h, the amount of folic acid-liposome uptake was more than 4 times higher than that of liposomes, reaching saturation at 0.45mg · mL -1. Excess folic acid competed to inhibit folate-liposome uptake. Phospholipase D or phosphatidylinositol-specific phospholipase C inhibits folic acid-liposome uptake by affecting folic acid receptors. Thus, the uptake of folate-liposomes by HeLa cells primarily through the folate receptor pathway: the ability of folate-rich or folate-rich liposomes to be up-regulated by highly active folate receptors was significantly increased compared to non-folate -containing liposomes.