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目的 :探讨全身及局部应用金钠多对豚鼠耳蜗微循环的影响 ,为临床治疗耳蜗微循环紊乱所致疾病提供实验依据。方法 :将 96只豚鼠随机分为圆窗膜用药组 (4 0只 )和静脉用药组 (5 6只 )。圆窗膜用药组又分为对照组 (7只 )、3.5 g/L组 (18只 )、0 .35 g/L组 (7只 )和0 .0 35 g/L组(8只 ) ;静脉用药组又分为对照组 (8只 )、2 .8mg/kg组 (12只 )、3.5mg/kg组 (15只 )、3.85mg/kg组 (10只 )和4 .2mg/kg组 (11只 )。圆窗膜用药组直接将药液或对照液 2 μl滴于圆窗龛 ;静脉用药组将药液或对照液经微泵均匀输入颈外静脉。采用激光多普勒流量仪 (LDF)监测耳蜗血流量 (CBF) ,用压力传感器及前置放大器记录平均动脉血压 (MABP) ,经A/D转换器同步输入计算机 ,供统计、分析。结果 :圆窗膜用药组中 ,对照组和 0 .0 35 g/L组CBF无明显变化 ;3.5 g/L组CBF下降 4 .95 %,0 .35 g/L组CBF下降4 .75 %,分别与对照组比较 ,差异均有极显著性意义 (均P <0 .0 1) ;MABP各组均无明显变化。静脉用药组中 ,对照组及 2 .8mg/kg组CBF无显著性变化。 3.5mg/kg组、3.85mg/kg组和4 .2mg/kg组CBF分别增加4 .5 3%、5 .2 1%及 6 .6 5 %,分别与对照组比较 ,差异均有极显著性意义 (均P <0 .0 1) ,且CBF变化与给药剂量之间存在明显的量效关系 (P <
Objective: To investigate the effects of systemic and topical application of sodium and sodium on cochlear microcirculation in guinea pigs, and to provide experimental evidence for the clinical treatment of diseases caused by disordered cochlear microcirculation. Methods: Ninety-six guinea pigs were randomly divided into round window membrane (60) and intravenous (56) groups. The round window membrane was divided into control group (7), 3.5 g / L group (18), 0.35 g / L group (7) and 0. 35 g / L group The intravenous drug group was divided into control group (8 rats), 2 .8 mg / kg group (12 rats), 3.5 mg / kg group (15 rats), 3.85 mg / kg group (10 rats) and 4.2 mg / kg group (11 only). Round window membrane medication group directly to the liquid or control solution 2 drops in round window niche; intravenous drug solution or control solution by micro-pump input jugular vein. The cochlear blood flow (CBF) was monitored by laser Doppler flowmeter (LDF). The mean arterial blood pressure (MABP) was recorded by pressure transducer and preamplifier. The data were simultaneously input to the computer through A / D converter for statistical analysis. Results: There was no significant change in the CBF of the control group and 0. 35 g / L group in the round window membrane treatment group. The CBF in the 3.5 g / L group decreased by 4.95% and the CBF in the 0. 35 g / L group decreased by 4.75% , Respectively, compared with the control group, the differences were significant (P <0.01); MABP no significant change in each group. Intravenous drug group, the control group and 2. 8mg / kg group CBF no significant change. The CBF of 3.5mg / kg, 3.85mg / kg and 4.2mg / kg groups increased by 4.53%, 5.21% and 6.65%, respectively, compared with the control group, the differences were significant (P <0.01), and there was a significant dose-response relationship between the change of CBF and the dose of administration (P <