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目的 :研究淫羊藿、枸杞子对老年大鼠线粒体DNA缺失、线粒体呼吸链酶复合体及ATP合成的影响。方法 :Wistar系雄性大鼠 ,幼年大鼠 (1月龄 )、青年大鼠 (4月龄 )每组均为 1 2只 ;老年大鼠 (2 2月龄 ,31只 ) ,随机分为对照组 (1 0只 )、淫羊藿组 (1 0只 )和枸杞子组 (1 1只 )。用聚合酶链反应 (PCR)、酶动力学和生物发光技术进行检测。结果 :淫羊藿、枸杞子可减少老年大鼠心、脑、骨骼肌组织线粒体DNA缺失 ,提高心、脑线粒体三磷酸腺苷(ATP)的合成和心、骨骼肌线粒体呼吸链复合酶Ⅳ活力及脑线粒体呼吸链复合酶Ⅰ活力 ;淫羊藿还可提高脑线粒体呼吸链复合酶Ⅳ活力和骨骼肌线粒体ATP的合成。结论 :淫羊藿、枸杞子对老年大鼠线粒体DNA的氧化损伤有保护作用
Objective : To study the effects of epimedium and gardenia on mitochondrial DNA deletion, mitochondrial respiratory chain enzyme complex and ATP synthesis in aged rats. METHODS: Wistar male rats were used. Young rats (1 month old) and young rats (4 months old) were used in 12 rats in each group; aged rats (22 months old and 31 rats) were randomly divided into control groups. Groups (10), Epimedium (10), and Scorpion (11). Detection was performed using polymerase chain reaction (PCR), enzyme kinetics, and bioluminescence techniques. RESULTS: Epimedium and Gardenia could reduce the mitochondrial DNA loss in heart, brain and skeletal muscles of aged rats, increase the synthesis of mitochondrial adenosine triphosphate (ATP) in heart and brain, and the mitochondrial respiratory chain complex enzyme IV activity in heart and skeletal muscle and brain mitochondria. Respiratory chain complex enzyme I activity; epimedium can also improve brain mitochondrial respiratory chain enzyme IV activity and skeletal muscle mitochondrial ATP synthesis. Conclusion : Epimedium and gardenia have protective effects on oxidative damage of mitochondrial DNA in aged rats