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目的了解沈阳地区慢性丙型肝炎患者的HCV基因分型情况,为丙型肝炎病毒的诊断和预防提供有力依据。方法根据丙型肝炎病毒RNA全序列,在5’-NCR区设计引物,通过套式PCR扩增,特异性产物测序分型。结果检测HCV感染样本75例,共检出4种基因型,分别为1a,lb,2a和3a型,其中1a占2.7%,1b占54.6%,2a占32%,3a型占10.7%。结论沈阳地区HCV基因型主要为1b型,其次为2a型,与中国其它地区HCV基因型分布比较一致。
Objective To understand the HCV genotyping in patients with chronic hepatitis C in Shenyang and to provide a strong evidence for the diagnosis and prevention of hepatitis C virus. Methods According to the complete sequence of hepatitis C virus RNA, primers were designed in the 5’-NCR region and amplified by nested PCR. The specific products were sequenced and classified. Results There were 75 HCV infection samples detected. Four genotypes were detected, which were 1a, 1b, 2a and 3a, respectively. Among them, 1a accounted for 2.7%, 1b accounted for 54.6%, 2a accounted for 32% and type 3a accounted for 10.7%. Conclusion The genotypes of HCV in Shenyang are mainly 1b, followed by 2a, which is consistent with the distribution of HCV genotypes in other parts of China.