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以六倍体裸燕麦578(大粒品种)和三分三(小粒品种)为亲本进行杂交,构建包含202个家系的F2遗传作图群体。由172个SSR标记构建出包含21个连锁群的遗传连锁图谱。采用复合区间作图对子粒性状进行QTL定位,共检测到17个控制子粒长度、宽度、千粒重的QTL位点。其中,6个与子粒长度相关的QTL位点表型的贡献率为0.70%~12.83%,5个与子粒宽度相关的QTL位点表型的贡献率为0.77%~12.92%,6个与子粒千粒重相关的QTL位点表型的贡献率为0.58%~10.64%。在这些QTLs中有4个的贡献率达到了10%以上,分别是与子粒长有关的qGL-2(12.83%)、与子粒宽有关的qGW-5(12.92%)以及与千粒重有关的qTGW-3(10.64%)和qTGW-4(10.05%),被认为是主效基因所在位点。而且qGL-2和qTGW-4位于连锁群的相同位置上。还发现第3号连锁群上AM1089~AM1512区段分别与子粒长度、宽度和千粒重相关,同时3号连锁群AM86-2~AM1044区间分别与子粒长度和千粒重相关,而位于第21号连锁群AM3217~AM965区段分别与子粒宽度和千粒重相关。这一研究为燕麦子粒性状的深入研究和相关标记开发以及分子辅助选择研究奠定了基础。
F2 population with 202 pedigrees was constructed by crosses of hexaploid naked oats 578 (big grain variety) and thirds (small grain variety) as parents. A total of 172 SSR markers were used to construct a genetic linkage map containing 21 linkage groups. QTL mapping of grain traits was carried out by using composite interval mapping. Seventeen QTLs controlling grain length, width and 1000-grain weight were detected. Among them, the contribution rate of six QTL loci phenotypes related to grain length was 0.70% ~ 12.83%, and the contribution rate of five QTL loci phenotypes related to grain width was 0.77% ~ 12.92% The contribution rate of 1000-grain weight QTL locus phenotype was 0.58% -10.64%. Among these QTLs, four contributed more than 10%, which were qGL-2 (12.83%) related to grain length, qGW-5 (12.92%) related to grain width and qTGW- 3 (10.64%) and qTGW-4 (10.05%) were considered as major loci. Moreover, qGL-2 and qTGW-4 are located in the same position in the linkage group. It was also found that AM1089 ~ AM1512 in linkage group 3 were related to the length, width and 1000-grain weight respectively. At the same time, AM86-2 ~ AM1044 in linkage group 3 were related to grain length and 1000-grain weight respectively, whereas AM3217 The AM965 segment is related to the grain width and 1000-grain weight, respectively. This research lays the foundation for further research on grain traits and related marker development and molecular aided selection in oat.