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该实验基于前期黑水缬草抗老年痴呆(AD)的作用,对其有效部位进行化学成分研究,采用75%乙醇加热回流的方法对黑水缬草药材进行提取,提取物经萃取和大孔吸附树脂柱色谱分离得到其抗AD的有效部位,采用硅胶、ODS柱色谱以及制备型HPLC等色谱法分离该有效部位并得到9个化合物(1~9),结合质谱及核磁等波谱技术,分别鉴定为6-hydroxy-7-(hydroxymethyl)-4-methy-lenehexahydrocyclopenta[c]pyran-1(3H)-one(1),suspensolide F(2),马钱子苷(3),α-莫诺苷(4),β-莫诺苷(5),patrinovalerosidate(6),野花椒苷A(7),(-)-angelicoidenol-2-O-β-D-glucopyranoside(8)和citroside A(9),其中化合物6~9为首次从缬草属植物中分离得到,进一步对化合物1~9进行体外抗AD活性研究发现化合物2和6对PC12神经元细胞损伤具有显著的保护作用。
The experiment was based on the role of prophylaxis of senile dementia (AD) in early stage, and the chemical constituents of its active site were studied. The medicinal materials of Valeriana officinalis were extracted by 75% The active site of anti-AD was isolated by adsorption resin column chromatography. The active site was separated by silica gel, ODS column chromatography and preparative HPLC, and 9 compounds (1 ~ 9) were obtained. Identification of 6-hydroxy-7- (hydroxymethyl) -4-methy lenehexahydrocyclopenta [c] pyran-1 (3H) -one (1), suspensolide F (-) - angelicoidenol-2-O-β-D-glucopyranoside (8) and citroside A (9) ). Among them, compounds 6 to 9 were isolated from Valeriana for the first time. Further studies on the anti-AD activity of compounds 1 to 9 showed that compounds 2 and 6 have a significant protective effect on PC12 neuronal cell injury.