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[目的]确定绞股蓝皂苷的最佳提取条件。[方法]制备标准曲线,用香草醛—高氯酸比色法测定绞股蓝皂苷的含量,通过正交试验研究浸提次数、浸提温度、乙醇浓度、料液比不同水平对绞股蓝皂苷提取率的影响。[结果]浸提次数、浸提温度、乙醇浓度、料液比对绞股蓝皂苷提取率的影响依次减小,浸提温度和浸提次数对提取绞股蓝皂苷的影响明显大于乙醇浓度和料液比。直观性分析表明,绞股蓝皂苷的最佳提取工艺为:70%乙醇溶液,料液比1∶12,在40℃下浸提3次,每次2 d。F检验表明,在3个乙醇浓度水平和3个料液比水平内提取绞股蓝皂苷的效果无显著差异,而温度和浸提次数各水平间的差异显著。[结论]在绞股蓝皂苷的提取过程中,不同提取条件对绞股蓝皂苷的提取率有很大的影响。
[Objective] The research aimed to determine the optimum extraction conditions of gypenosides. [Method] The standard curve was prepared. The content of gypenosides was determined by vanillin - perchloric acid colorimetric method. The orthogonal experiment was used to study the extraction times, extraction temperature, ethanol concentration, influences. [Result] The effects of extraction times, extraction temperature, ethanol concentration and solid-liquid ratio on the extraction yield of gypenosides decreased in turn. The effects of extraction temperature and extraction times on the extraction of gypenosides were significantly greater than that of ethanol and solid-liquid ratio. Intuitive analysis showed that the optimum extraction process of Gypenosides: 70% ethanol solution, solid-liquid ratio 1:12, leaching three times at 40 ℃, each 2 d. F test showed that there was no significant difference in the effect of extracting gypenoside between the three ethanol concentration levels and the three solid-liquid ratio levels, while the difference between the temperature and the number of extraction stages was significant. [Conclusion] Different extraction conditions had a great influence on the extraction rate of gypenosides during the extraction of gypenosides.