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目的:本研究采用UPLC建立了同时测定贵州产荭草药材中儿茶素、异荭草素、荭草素、槲皮素-3-O-(2″-O-α-鼠李糖)-β-葡萄糖醛酸、花旗松素、木犀草苷、槲皮苷、山柰素-3-O-β-D-葡萄糖苷8种指标成分的含量。方法:ACQU-ITY UPLC BEHC18色谱柱(2.1 mm×150 mm,1.7μm),乙腈-0.1%磷酸水溶液为流动相,流速为0.30 mL.min-1,梯度洗脱,检测波长260 nm,柱温45℃。试验分别对线性、仪器精密度、重复性、中间精密度、准确性等项目进行了验证。结果:被测定的8个化合物分别在选定的范围内线性关系良好(r≥0.999 8),平均加样回收率在97.6%～102.5%,RSD 1.9%～4.7%(n=6)。结论:贵州产荭草的化学成分基本相同,但被测定的成分含量有一定差别,在8月份采收的药材中被测成分的含量较高且较为稳定。
OBJECTIVE: To establish a method for the simultaneous determination of catechin, isoorientin, glycyrrhizin, quercetin -3-O- (2 "-O-α- rhamnose) β-glucuronic acid, Taxifolin, luteolin, quercetin and behen-3-O-β-D-glucoside.Methods: ACQU-ITY UPLC BEHC18 column (2.1 mm × 150 mm, 1.7 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution with a flow rate of 0.30 mL · min-1 and gradient elution. The detection wavelength was 260 nm and the column temperature was 45 ° C. The linearity, , Repeatability, intermediate precision and accuracy were tested.Results: The eight compounds tested showed good linearity (r≥0.999 8) within the selected range respectively, and the average recoveries were in the range of 97.6% 102.5%, and RSD 1.9% -4.7% (n = 6) .Conclusion: The chemical constituents of Gentiana officinalis in Guizhou are basically the same, but the contents of the constituents determined are different. The content of the tested components in the medicinal materials collected in August Higher and more stable.