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目的探讨EB病毒(EBV)抗体检测在诊断EBV相关性传染性单核细胞增多症(IM)中的意义。方法北京儿童医院2005年1月至2006年12月住院治疗诊断为IM的患儿220例,人院后采集单份血清标本,采用间接免疫荧光法检测抗EBV-CA-IgM抗体、抗EBV-CA-IgG抗体、抗EBV-EA-IgG抗体、抗EBV-NA-IgG抗体及EBV-CA-IgG抗体亲和力。结果220例诊断IM患儿中,各种抗EBV抗体的阳性率分别为:抗EBV-CA-IgG 100%,抗EBV-CA-IgM 95.9%,抗EBV-EA-IgG 79.5%,抗EBV-NA-IgG 4.1%。抗EBV-CA-IgG抗体为低亲和力的患儿204例(92.7%)。186例患儿(84.6%)EBV抗体种类表现为EBV-CA-IgM和EBV-CA-IgG均阳性,EBV-NA-IgG阴性且EBV- CA-IgG为低亲和力抗体,是IM病例中抗EBV抗体反应的主要类型。结论4种抗EBV抗体(CA-IgG、CA-IgM、EA-IgG、NA-IgG及CA-IgG亲和力)检测结果能够为临床提供更多信息,从而更加准确地判断EBV感染的时期,对于IM诊断提供更可靠的确诊依据。
Objective To investigate the significance of detecting Epstein-Barr virus (EBV) antibody in the diagnosis of EBV-associated infectious mononucleosis (IM). Methods 220 children diagnosed as IM from Beijing Children’s Hospital from January 2005 to December 2006 were enrolled in this study. Single serum samples were collected after hospitalization. Anti-EBV-CA-IgM antibody was detected by indirect immunofluorescence, CA-IgG antibody, anti-EBV-EA-IgG antibody, anti-EBV-NA-IgG antibody and EBV-CA-IgG antibody. Results The positive rates of various anti-EBV antibodies in 220 infants diagnosed with IM were 100% for anti-EBV-CA-IgG, 95.9% for anti-EBV-CA-IgM, 79.5% NA-IgG 4.1%. 204 patients (92.7%) had low-affinity EBV-CA-IgG antibody. EBV-CA-IgM and EBV-CA-IgG were positive in EBV-NA-IgG and EBV-CA-IgG as low-affinity antibody in 186 patients (84.6% The main type of antibody response. Conclusion The results of four anti-EBV antibodies (CA-IgG, CA-IgM, EA-IgG, NA-IgG and CA-IgG affinity) can provide more information for clinical diagnosis of EBV infection. Diagnosis provides a more reliable basis for diagnosis.