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目的:检测IgG受体(FcγR)在炎症细胞因子刺激的人脐静脉内皮细胞(HUVEC)上的表达。方法:采用ELISA、免疫组化染色法、免疫荧光法和RT-PCR等方法,检测FcγRII及其表达。结果:未经细胞因子刺激的正常HU-VEC可表达低水平的FcγRIIa;经2×105U/L TNF-α和1×105U/L IFN-γ联合刺激3d后,FcγRIIa的表达提高16倍(P<0.01)。免疫荧光法检测显示,TNF-α和IFN-γ诱导的FcγRIIa表达于HUVEC表面。RT-PCR结果显示,FcγRIIamRNA表达量在TNF-α和IFN-γ刺激24h后有明显提高。结论:TNF-α和IFN-γ可通过调节FcγRIIa的转录和翻译,增强其在HUVEC上的表达。FcγRIIa的表达可能与血管炎条件下,免疫复合物在血管上的特异性定位有关。
OBJECTIVE: To detect the expression of IgG receptor (FcγR) on inflammatory cytokines stimulated human umbilical vein endothelial cells (HUVECs). Methods: The expression of FcγRII and its receptor were detected by ELISA, immunohistochemistry, immunofluorescence and RT-PCR. Results: The normal HU-VEC without cytokine stimulation could express low level of FcγRIIa. After stimulated with 2 × 105U / L TNF-α and 1 × 105U / L IFN-γ for 3 days, the expression of FcγRIIa increased by 16 folds (P <0.01). Immunofluorescence assay showed that FcγRIIa induced by TNF-α and IFN-γ was expressed on the surface of HUVEC. The results of RT-PCR showed that the expression of FcyRIIamRNA was significantly increased after TNF-|Á and IFN-|Ã stimulation 24h. Conclusion: TNF-α and IFN-γ can enhance their expression on HUVEC by regulating the transcription and translation of FcγRIIa. The expression of FcyRIIa may be related to the specific localization of immune complexes in the blood vessels under the condition of vasculitis.