研究选取9个优良玉米品种的自交系,以幼胚为外植体,以N6、MS培养基为基本培养基,诱导愈伤组织,探讨基因型、培养基类型、外植体大小、激素浓度等因素对愈伤组织诱导率的影响,分析不同基因型愈伤组织生长状态的差异,对诱导成功的愈伤组织进行分化和再生。研究结果表明,基因型对愈伤组织诱导率起决定作用,且基因型和培养基之间存在相互作用;外植体大小在1.5～2.0 mm时的幼胚较适合诱导胚性愈伤组织;2,4-D对玉米幼胚愈伤组织的诱导形成起关键作用,浓度在2.0～2.5 mg/L时大多能诱导出胚性愈伤组织。 In this study, nine inbred lines of maize varieties were selected, and immature embryos were used as explants. N6 and MS medium were used as basic medium to induce callus. Genotype, medium type, explant size, hormone Concentration and other factors on the callus induction rate, analysis of different genotypes of callus differences in growth status, callus induction of successful differentiation and regeneration. The results showed that the genotypes played a decisive role on the induction rate of callus, and there was interaction between genotypes and culture medium. Immature embryos of explants with sizes of 1.5-2.0 mm were suitable for inducing embryogenic callus. 2,4-D played a key role in inducing callus induction of maize immature embryos, most of which induced embryogenic callus at concentrations of 2.0-2.5 mg / L.