为了检测小细胞肺癌(SCLC)是否有特殊的染色体变化,作者从6例未经治疗和10例系统接受治疗的SCLC 患者采集原发肿瘤细胞、骨髓、胸膜渗出液,转移结节和皮下肿瘤的标本建立16株细胞株.其方法是将细胞悬液和小细胞聚集物放入RPMI1680培养基内,加入10%热灭活胎牛血清,把约有1×10细胞培养物放入75cm 培养瓶内,在5% CO_2 内,37℃下孵育,生长4—6个月.作者分析了16株SCLC 组 To test whether there is a specific chromosome change in small cell lung cancer (SCLC), the authors collected primary tumor cells, bone marrow, pleural effusions, metastatic nodules, and subcutaneous tumors from 6 untreated and 10 systemically treated SCLC patients. Specimens were used to establish 16 cell lines. The method was to place the cell suspension and small cell aggregates in RPMI1680 medium, add 10% heat-inactivated fetal bovine serum, and place approximately 1×10 cell culture in 75 cm culture. Incubate in 5% CO 2 at 37°C for 4-6 months. The authors analyzed 16 SCLC groups.