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采用逆转录—多聚酶链反应(RT/PCR)法检测了泰安地区46例输血后丙型肝炎(PTH-C)患者的血清HCV-RNA,对其中34例HCV-RNA阳性者的PCR产物用限制性长度多态性分析法(RFLP)进行了HCV基因分型。结果HCV-Ⅱ型检出率为73.5%,HCV-Ⅲ型检出率为11.8%;HCVⅡ/HCVⅢ型混合感染率为14.7%,未发现Ⅰ、Ⅳ和Ⅴ型感染。
HCV-RNA was detected in 46 patients with transfusion of hepatitis C (PTH-C) after transfusion by reverse transcription-polymerase chain reaction (RT / PCR) method. PCR products of 34 HCV-RNA positive patients were restricted Sex length polymorphism (RFLP) was used to genotype HCV. Results The detection rate of HCV-Ⅱ was 73.5% and that of HCV-Ⅲ was 11.8%. The infection rate of HCVⅡ / HCV Ⅲ was 14.7%. No infection of type Ⅰ, Ⅳ and Ⅴ was found.