In the three-phase traffic flow studies, the traffic flow characteristic at the bottleneck section is a hot spot in the aca-demic field. The controversy about the characteristics of the synchronized flow at bottleneck is also the main contradiction between the three-phase traffic flow theory and the traditional traffic flow theory. Under the framework of three-phase traffic flow theory, this paper takes the on-ramp as an example to discuss the traffic flow characteristics at the bottleneck section. In particular, this paper mainly conducts the micro-analysis to the effect of lane change under the two lane conditions, as well as the effect of the on-ramp on the main line traffic flow. It is found that when the main road flow is low, the greater the on-ramp inflow rate, the higher the average speed of the whole road section. As the probability of vehicles entering from the on-ramp increases, the flow and the average speed of the main road are gradually stabilized, and then the on-ramp inflow vehicles no longer have a significant impact on the traffic flow. In addition, this paper focuses on the velocity distur-bance generated at the on-ramp, and proposes the corresponding on-ramp control strategy based on it, and the simulation verified that the control strategy can reasonably control the traffic flow by the on-ramp, which can meet the control strategy requirements to some extent.