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目的研究SOS/umu试验诱导活性的影响因素,为建立规范化试验方法提供依据。方法比较了SOS/umu试验中增菌器皿、菌株保存方式对umu C诱导活性的影响,探讨了菌株生物学特性、预培养温度、溶剂选择、受试物本身等对试验结果可能产生的影响;同时选取了3种已知致突变剂,进行了SOS/umu测试,并与文献报道的Ames试验结果进行了比较。结果 SOS/umu测试结果显示,在试验增菌预培养时,底面积较大的三角烧瓶相较于底面积较小的试管,其对数生长期微生物增殖数量明显升高,是比较适宜的培养器皿;液氮保存的菌株对甲基磺酸甲酯(MMS)的诱导活性明显高于平板保存菌株;对于3种已知致突变剂,2种方法均能检测出其遗传毒性,SOS/umu试验阳性最低检出剂量均明显低于Ames试验,相同剂量下的诱导比率均大于Ames试验,显示SOS/umu试验敏感性略大于Ames试验。结论若严格控制试验条件,完善试验细节,使其标准化,SOS/umu试验有望成为规范的检测大规模环境样品遗传毒性的初筛和监测的方法。
Objective To study the influencing factors of the induction activity of SOS / umu test and provide the basis for the establishment of standardized test methods. Methods The effects of enrichment ware and strain preservation methods on the induction of umu C in SOS / umu test were compared. The possible influence of the strain biological characteristics, preincubation temperature, solvent selection and the test substance on the test results were discussed. At the same time, three kinds of known mutagens were selected for SOS / umu test and compared with Ames test results reported in the literature. Results The results of SOS / umu test showed that the number of microbial proliferation in logarithmic growth phase was significantly higher in the conical flask with larger bottom area than that in the tube with the smaller bottom area when pre-cultured in the experiment, Vessel; the induced activity of methyl methanesulfonate (MMS) in liquid nitrogen was significantly higher than that of plate-stored strain; for the three known mutagens, the genotoxicity of SOS / umu The lowest test positive test dose was significantly lower than the Ames test, the same dose of the induction ratio were greater than the Ames test, SOS / umu test sensitivity was slightly greater than the Ames test. Conclusion SOS / umu test is expected to become the standard method for the screening and monitoring of genotoxicity in large-scale environmental samples if the test conditions are strictly controlled and the details of the test are standardized and standardized.