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目的研究丹参川芎嗪注射液对大鼠脑基底动脉的扩张及其机制。方法利用小血管张力测量系统研究丹参川芎嗪注射液对经高K~+、苯肾上腺素(PE)和血栓素受体激动剂(U46619)预收缩的血管环的扩张作用,并研究在不含钙的缓冲液中丹参川芎嗪注射液对PE、氯化钙(CaCl_2)及咖啡因收缩血管的影响。利用激光共聚焦显微镜观察丹参川芎嗪注射液对大鼠肠系膜血管平滑肌钙离子浓度的影响。结果 15~80 m L·L~(-1)丹参川芎嗪注射液可以浓度依赖性地降低经高K~+、PE和U46619预收缩的大鼠脑基底动脉;在不含钙的缓冲液中,丹参川芎嗪注射液(15,45,80 m L·L~(-1))可以抑制CaCl_2诱导的血管收缩,对PE及咖啡因诱导的血管收缩无影响;丹参川芎嗪注射液(45,60,80 m L·L~(-1))对高K~+诱导的大鼠肠系膜血管环收缩及血管壁荧光增强有不同程度的抑制作用。结论丹参川芎嗪注射液可以浓度依赖性地舒张大鼠脑基底动脉,抑制血管平滑肌钙离子通道是主要作用机制之一,主要表现为抑制细胞外的钙离子进入。
Objective To study the dilatation of basilar artery of Salvia miltiorrhiza Bunge in rats and its mechanism. Methods The small vessel tension measurement system was used to study the dilatation of Salviae miltiorrhizae ligustrazine injection on the vascular rings precontracted by high K +, phenylephrine (PE) and thromboxane receptor agonist (U46619) Effect of Salviae Miltiorrhizae and Ligustrazine Injection on PE, Calcium Chloride (CaCl2) and Caffeine Systolic Blood Vessel in Calcium Buffer. Effects of Salvia miltiorrhiza Ligustrazine Injection on the Concentration of Calcium in Rat Mesentery Vascular Smooth Muscle by Laser Scanning Confocal Microscopy. Results 15 ~ 80 m L · L -1 Salviae miltiorrhizae ligustrazine injection could significantly reduce the basilar artery of rat precontracted by high K ~ +, PE and U46619 in a concentration-dependent manner; in the calcium-free buffer , Salviae miltiorrhizae ligustrazine injection (15, 45, 80 m L·L -1) can inhibit CaCl 2 -induced vasoconstriction and had no effect on PE and caffeine-induced vasoconstriction. Salviae miltiorrhizae ligustrazine injection (45, 60 and 80 m L · L -1) inhibited the contraction of mesenteric vessels and the enhancement of fluorescence in vessel wall induced by high K +. Conclusion Salviae miltiorrhizae injection can relax the basilar artery of rat brain in a concentration-dependent manner and inhibit the calcium channel of vascular smooth muscle, which is one of the main mechanisms. It mainly inhibits the extracellular Ca2 + entry.