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本研究探讨磷酸胞苷酰寡脱氧核苷酸(CpG-ODN)和白介素-2(IL-2)联合刺激培养慢性淋巴细胞白血病(CLL)细胞的遗传学特征。对115例CLL患者的外周血(或骨髓)标本,应用CpG-ODN DSP30联合IL-2刺激培养CLL细胞72小时,常规收获细胞制备染色体并进行R显带分析,探讨CLL的染色体核型特征。结果表明:115例CLL患者中,染色体分析成功率(可分析分裂相≥20个)为74.8%,异常核型检出率为58.1%。检测出一种染色体异常21例(24.4%),2种染色体异常6例(7.0%),复杂核型23例(26.7%),其中高度复杂核型9例(10.5%)。在86例患者中共检出102种163个异常,其中数目异常116个(71.2%),结构异常47个(28.8%),最常见的染色体数目异常是+12(14.0%),结构异常是15q+(5.8%)。结论:大多数CLL患者具有细胞遗传学异常,其中染色体数目异常明显多于结构异常。采用DSP30联合IL-2可以有效的增加CLL细胞的分裂相,提高CLL细胞染色体异常检出率。
This study was designed to investigate the genetic characteristics of CLL cells cultured with CpG-ODN and IL-2. The peripheral blood (or bone marrow) samples of 115 patients with CLL were cultured in CLL cells with CpG-ODN DSP30 combined with IL-2 for 72 hours. The chromosomes were routinely harvested and analyzed by R banding to investigate the chromosomal karyotype characteristics of CLL. The results showed that in 115 cases of CLL, the success rate of chromosomal analysis (analysis of schizophrenia ≥ 20) was 74.8%, the detection rate of abnormal karyotype was 58.1%. There were 21 cases (24.4%) with one chromosomal abnormality, 6 cases (7.0%) with 2 chromosomal abnormalities and 23 cases (26.7%) with complex karyotypes, of which 9 cases (10.5%) were highly complex karyotypes. A total of 102 abnormalities of 163 were detected in 86 patients, of which 116 (71.2%) were abnormal, 47 (28.8%) were abnormal, and the most common chromosomal abnormality was +12 (14.0%). The structural abnormalities were 15q + (5.8%). CONCLUSIONS: Most CLL patients have cytogenetic abnormalities in which there are significantly more chromosomes than structural abnormalities. The combination of DSP30 and IL-2 can effectively increase the cleavage phase of CLL cells and increase the detection rate of chromosomal abnormalities in CLL cells.