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目的:建立同时测定林蛙油中α-亚麻酸、亚油酸和油酸含量的HPLC方法。方法:Alltima C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(80∶20)为流动相,流速1 mL.min-1,柱温30℃,检测波长203 nm。结果:α-亚麻酸在4.76～152.16 mg.L-1(r=1),亚油酸在15.36～491.59 mg.L-1(r=0.999 8),油酸在47.28～1512.80 mg.L-1(r=0.999 9)呈良好的线性关系;平均回收率α-亚麻酸为95.8%,RSD 1.79%(n=6);亚油酸为98.6%,RSD 1.32%(n=6);油酸为99.3%,RSD 1.59%(n=6)。结论:该法操作简便,结果准确,专属性强,重复性好,可作为林蛙油质量控制的参考。
Objective: To establish an HPLC method for simultaneous determination of α-linolenic acid, linoleic acid and oleic acid in Rana oil. METHODS: Alltima C18 column (4.6 mm × 250 mm, 5 μm) was used with a mobile phase of acetonitrile-0.1% phosphoric acid (80:20) at a flow rate of 1 mL · min-1 and a column temperature of 30 ℃. Results: The results showed that α-linolenic acid had a significant difference between 4.76 and 152.16 mg.L-1 (r = 1), linoleic acid between 15.36 and 491.59 mg.L-1 (r = 0.999 8), oleic acid between 47.28 and 1512.80 mg.L- (R = 0.999 9). The average recoveries of α-linolenic acid were 95.8%, RSD 1.79% (n = 6), linoleic acid was 98.6% and RSD was 1.32% (n = 6) Acid 99.3%, RSD 1.59% (n = 6). Conclusion: The method is simple, accurate, specific and reproducible. It can be used as a reference for the quality control of Rana chensinensis.