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目的建立清火栀麦胶囊的质量标准。方法采用薄层色谱法对清火栀麦胶囊中的穿心莲、麦冬鉴别分析;用高效液相色谱法测定栀子中栀子苷的含量,采用Nova-pakC184μm,3.9mm×l50mm色谱柱,流动相:甲醇-水2575,检测波长:240nm。结果在薄层色谱中均能检出穿心莲、麦冬;栀子苷在0.228~1.14lug范围内呈良好线性关系r=0.9999,平均回收率为99.06%,RSD为1.28%n=5。结论该方法简便准确,重现性好。
Objective To establish the quality standard of Qinghuo buckwheat capsules. Methods TLC method was used to identify the concentrations of Andrographis paniculata and Ophiopogon japonicus in Qing Huo Yemai Capsules. The content of jasminoidin in Dangzi was determined by high performance liquid chromatography. Nova-pakC184μm and 3.9mm×l50mm columns were used. Mobile phase: methanol-water 2575, detection wavelength: 240 nm. Results Andrographis paniculata and Ophiopogon japonicus could be detected in TLC. The geniposide showed good linearity in the range of 0.228~1.14gg, r=0.9999, and the average recovery was 99.06%. The RSD was 1. .28% n=5. Conclusion This method is simple, accurate and reproducible.