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目的:观察孕鼠被动吸烟对仔鼠炎症相关信号通路及炎症因子及认知功能的影响,探讨炎症反应与认知功能的相关性。方法:按照母鼠怀孕先后顺序随机分成两组,采用孕鼠被动吸烟法制造仔鼠发育迟缓模型,均灌胃蒸馏水。自然分娩后喂养仔鼠1个月,对仔鼠采用Morris水迷宫法测试空间定位学习能力和空间记忆水平,并用免疫组化法及RT-qRCR检测P50、P65及TNF-α、IL-1β的表达。结果:与正常组比较,模型组逃逸潜伏期延长,垮台次数减少(P<0.05);免疫组化及RT-q PCR检测大致相同,与正常组比较,模型组P50、P65及IL-1β、TNF-α表达高于正常组(P<0.05)。结论:孕期被动吸烟可导致神经组织产生炎症反应,脑内P50、P65及IL-1β、TNF-α表达上调,学习记忆能力下降。
Objective: To observe the effect of passive smoking on the inflammatory signal pathways, inflammatory factors and cognitive function of pregnant rats, and to explore the correlation between inflammatory reaction and cognitive function. Methods: According to the order of maternal pregnancy, the rats were randomly divided into two groups. The model of passive growth was established by passive smoking pregnant rats, all of which were infused with distilled water. After natural delivery, the pups were fed for one month. Morris water maze test was used to test the spatial learning ability and spatial memory of the offspring. Immunohistochemistry and RT-qRCR were used to detect the expression of P50, P65, TNF-α and IL-1β expression. Results: Compared with normal group, escape latency of model group was longer than that of normal group (P <0.05), and immunohistochemistry and RT-q PCR were similar. Compared with normal group, P50, P65 and IL-1β, -α expression was higher than the normal group (P <0.05). CONCLUSION: Passive smoking during pregnancy leads to inflammatory reaction in nerve tissue. The expression of P50, P65, IL-1β and TNF-α in the brain is upregulated, and learning and memory abilities decrease.