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目的探讨人类免疫缺陷病毒Ⅰ型(HIV-1)B′亚型感染者人类白细胞抗原(HLA)-A、-B、-C位点等位基因的分布及其对病毒载量的影响。方法对146例HIV-1B′亚型感染者采用聚合酶链反应-序列特异性引物(PCR-SSP)扩增方法进行HLA-A、-B、-C位点等位基因检测,计算各位点等位基因的频率及其对感染者病毒载量的影响。结果在146例HIV-1B′亚型感染者中分别检出HLA-A位点14个等位基因,-B位点24个等位基因,-Cw位点12个等位基因,其中频率大于0.1的等位基因为HLA-A*02,-A*11,-A*24,-A*30,-B*13,-B*40,-B*51,-Cw*03,-Cw*06,-Cw*08;HLA-A、-B、-C等位基因纯合子携带者的病毒载量高于杂合子(P=0.0013);HLA-A*03(P=0.0314)、-A*30(P=0.0072)、-B*13(P=0.0087)、-Cw*06(P=0.0145)等位基因携带者具有较低病毒载量。结论 HIV-1B′亚型感染者HLA-A*03、-A*30、-B*13、-Cw*06等位基因与低病毒载量相关。
Objective To investigate the distribution of human leukocyte antigen (HLA) -A, -B, -C loci alleles in human immunodeficiency virus type 1 (HIV-1) B subtypes and their effect on viral load. Methods The alleles of HLA-A, -B, -C loci were detected by polymerase chain reaction-sequence specific primers (PCR-SSP) in 146 HIV-1B subtypes. The frequency of alleles and their effect on the viral load of infected persons. Results Among 146 HIV-1B subtypes, 14 alleles were detected in HLA-A locus, 24 alleles in B locus and 12 alleles in -Cw locus, of which frequency was greater than The alleles of 0.1 were HLA-A * 02, -A * 11, -A * 24, -A * 30, -B * 13, -B * 40, -B * 51, -Cw * 03, -Cw * (P = 0.0013); HLA-A * 03 (P = 0.0314), -A * 30 (P = 0.0072), - B * 13 (P = 0.0087), - Cw * 06 (P = 0.0145) allele carriers had lower viral load. Conclusion HLA-A * 03, -A * 30, -B * 13 and -Cw * 06 alleles are associated with low viral load in HIV-1B subtype.