Through the fluorescence and ultraviolet- visible spectrophotometry we have studied the action of ce- foperazone sodium and worfloxacin, two kinds of medicine with albumin, and examined the equal efficiency quality of fluorescence enhancement and fluorescence quenching equa- tion. The result shows the equal efficiency of calculating dis- sociation constant. In this article, we obtain the dissociation constants of the action of the two kinds of medicine with bo- vine serum albumin, energy- transfer efficiency, the distance of donor-acceptor and quenching constants. The above shows that the action is not caused by dynamic collision but by the binding of medicine and albumin. This article specially points out that the [Mt]/[Pt], which is the ratio of the maxi- mum mol concentration of the quenching agent (medicine) to fluorescent (albumin) mol concentration when the quenching ends, is very important because it is the maximum binding number of quenching agent molecule to a fluorescent mole- cule. There is no more quenching after adding more quench- ing agent beyond this quantity to fluorescent solution. This quantity is determined directly and strictly by experiment, so is also of important significance when discussing quenching efficiency and binding situation of donor-acceptor. Through the fluorescence and ultraviolet-visible spectrophotometry we have studied the action of ce- foperazone sodium and worfloxacin, two kinds of medicine with albumin, and examined the equal efficiency quality of fluorescence enhancement and fluorescence quenching equa- tion. of calculating dis- sociation constant. In this article, we obtain the dissociation constants of the action of the two kinds of medicine with bo- vine serum albumin, energy-transfer efficiency, the distance of donor-acceptor and quenching constants. that the action is not caused by dynamic collision but by the binding of medicine and albumin. This article specifically points out that the [Mt] / [Pt], which is the ratio of the maxi- mum mol concentration of the quenching agent (medicine ) to fluorescent (albumin) mol concentration when the quenching ends, is very because because it is the maximum binding number of quenching agent molecule to a fluorescent mole This quantity is determined directly and strictly by experiment, so is also important for when discussing quenching efficiency and binding situation of donor-acceptor.