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[目的]对陆地棉PHYB基因有一个全面的认识和生物信息学分析。[方法]在陆地棉异源四倍体标准系TM-1基因组数据库中共鉴定获得2个PHYB基因,这2个基因分别分布在A10和D10亚基因组。利用生物信息学方法对这两个基因进行初步分析。[结果]棉花的PHYB具有其他植物PHYB相同的基序和结构域,不管是数目还是分部位置都比较一致;不同植物的PHYB氨基酸序列同源性比对及构建的进化树都显示棉花的PHYB同可可的在同源性和进化关系上都是最高的,同水稻、玉米等单子叶植物的同源性及进化关系较低;基因结构的比对结果同样显示植物PHYB基因在进化上比较保守;棉花PHYB存在几十个可能磷酸化的位点,这些位点的磷酸化作用可能影响光敏色素的功能及其介导的信号通路。[结论]该研究结果为陆地棉PHYB基因的克隆和功能研究提供了理论基础。
[Objective] The research aimed to provide a comprehensive understanding and bioinformatics analysis of PHYB gene in upland cotton. [Method] Two PHYB genes were identified in TM-1 genomic database of Upland Cotton (Upland), two genes were distributed in A10 and D10 subgenomes, respectively. Bioinformatics methods were used to analyze the two genes. [Result] The PHYB of cotton had the same motif and domain of PHYB in other plants, no matter the number or location of branches. The comparison of PHYB amino acid sequence homology and the constructed phylogenetic tree showed that the PHYB Homology and evolutionary relationship with cocoa are the highest, with homology and evolution of rice, maize and other monocotyledons low correlation; gene structure comparison results also show phytosph PHYB gene evolutionarily conservative There are several dozens of possible phosphorylation sites in cotton PHYB. The phosphorylation of these sites may affect the function of phytochromes and their signaling pathways. [Conclusion] The results provided the theoretical basis for the cloning and function of PHYB gene in upland cotton.