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目的:建立高效液相色谱法测定抗感解毒颗粒中葛根素的含量。方法:采用Diamonsil-C_(18)(250 mm×4.6 mm,5μm)色谱柱;甲醇-水(25:75)为流动相;柱温为30℃;流速为1.0 ml·min~(-1);检测波长为250nm。结果:葛根素进样量在0.0800～0.6420μg间呈良好的线形关系;平均加样回收率为99.6%(RSD=1.3%)。结论:本方法准确可行,可用于本品的质量控制。
Objective: To establish a HPLC method for the determination of puerarin in anti-detoxification and granules. Methods: A Diamonsil-C_(18) (250 mm×4.6 mm, 5 μm) column was used. The mobile phase was methanol-water (25:75). The column temperature was 30°C and the flow rate was 1.0 ml·min -1. Detection wavelength is 250nm. RESULTS: The puerarin injection showed a good linear relationship between 0.0800 and 0.6420 μg. The average recovery was 99.6% (RSD = 1.3%). Conclusion: This method is accurate and feasible and can be used for quality control of this product.