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目的研究黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞的毒性。方法 75%乙醇回流提取得黄药子提取物,高、中、低剂量作用于Caco-2细胞,以黄药子醇提物Caco-2细胞摄取液作用于HL-7702和HepG2细胞,进行细胞活性实验,测定生化指标ALT、AST、GSH-PX和MDA值。结果与对照组比,高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用后,HL-7702和HepG2细胞的存活率显著降低(P<0.01);高剂量组黄药子醇提物Caco-2细胞摄取液作用72 h后,HL-7702细胞上清液中ALT、AST显著升高(P<0.01);高剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后,HepG2细胞上清液中ALT、AST显著升高(P<0.01)。高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后,HL-7702和HepG2细胞上清液中MDA显著升高,GSH-PX显著降低(P<0.01)。结论黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞有毒性。
Objective To study the toxicity of xanthium alcohol extract Caco-2 cells to HL-7702 and HepG2 cells. Methods The extract of Radix Astragali was extracted with 75% ethanol and the cells were treated with high, medium and low doses of Caco-2 and HL-7702 and HepG2 cells with the extracts of Caco-2. Biochemical indicators ALT, AST, GSH-PX and MDA values. Results Compared with the control group, the survival rates of HL-7702 and HepG2 cells in HL-7702 and HepG2 cells were significantly decreased (P <0.01) after high-dose and medium-dose Xanthium alcohol extract Caco-2 cells uptake; Caco-2 cells in 72 hours after treatment, HL-7702 cells supernatant ALT, AST was significantly increased (P <0.01); high-dose Xanthium alcohol extract Caco-2 cells in 48 h and 72 h After, HepG2 cell supernatant ALT, AST was significantly increased (P <0.01). The contents of MDA in HL-7702 and HepG2 supernatants were significantly increased and GSH-PX was significantly decreased (P <0.01) after treated with Caco-2 cells in high dose and medium dose groups for 48 h and 72 h. . Conclusion The extract of Xanthium alcohol Caco-2 cells is toxic to HL-7702 and HepG2 cells.