论文部分内容阅读
目的了解人博卡病毒(HBoV)在长沙地区急性下呼吸道感染住院儿童中的流行情况及临床特征,比较分析其区域流行特点。方法收集2007年9月—2008年3月长沙地区773份急性下呼吸道感染住院儿童鼻咽抽吸物(nasopharyngeal aspirates,NPAs)样本进行HBoV病毒NP1基因检测,将PCR阳性产物测序,并将所测序列在GenBank中进行比较分析。结果 773例样本中,HBoV阳性例数87例,HBoV感染患儿年龄为18 d～64个月,冬春季节流行,主要的临床诊断与症状是支气管肺炎与咳嗽;所测序列与GenBank公布的不同地区的HBoV NP1基因核苷酸序列同源性达99.53%,氨基酸序列同源性达99.8%,对核苷酸序列作基因进化树分析显示属于1种基因型。结论长沙地区部分急性下呼吸道感染儿童与人博卡病毒有关,且HBoV感染在低年龄组的儿童中更常见;一种基因型在长沙地区流行。
Objective To understand the prevalence and clinical characteristics of human Boca Virus (HBoV) in hospitalized children with acute lower respiratory tract infection in Changsha and to compare the epidemiological characteristics of HBoV. Methods A total of 773 nasopharyngeal aspirates (NPAs) samples from hospitalized children with acute lower respiratory tract infection in Changsha were collected from September 2007 to March 2008 in Dalian. The NP1 gene of HBoV virus was detected. The PCR positive products were sequenced. The sequences were compared in GenBank. Results Among the 773 samples, 87 cases were positive for HBoV, and the age of children infected by HBoV was 18 days to 64 months. The epidemic occurred in winter and spring. The main clinical diagnosis and symptoms were bronchopneumonia and cough. The homology of nucleotide sequence of HBoV NP1 gene was 99.53% and the homology of amino acid sequence was 99.8% in different regions. Phylogenetic tree analysis showed that the nucleotide sequence of HBoV NP1 gene belonged to one genotype. Conclusions Some children with acute lower respiratory tract infection in Changsha area are associated with human BACA, and HBoV infection is more common in children of the lower age group. One genotype is prevalent in Changsha.