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目的探讨云南大麻DNA的提取及检测方法。方法运用改进的SDS微量法提取大麻总DNA,对所得DNA进行PCR检测。结果用所筛选到的大麻引物检测了云南大麻的DNA遗传标记特征,图谱清晰,结果稳定。结论本方法能有效提取并检测大麻DNA,为实现大麻的品种鉴定、遗传多态性研究及来源追踪提供有价值的科学依据。
Objective To explore the method of DNA extraction and detection in Yunnan hemp. Methods The total DNA of cannabis was extracted by the improved SDS microdialysis method. The DNA was detected by PCR. Results The genetic markers of Yunnan hemp were detected with the selected hemp primers and the results were clear and the results were stable. Conclusion This method can effectively extract and test the DNA of cannabis. It provides valuable scientific evidence for the identification of varieties, genetic polymorphism and source tracing of cannabis.