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用T细胞克隆法分析了用低剂量和高剂量X射线照射PHA刺激后处于第1个G_1期人淋巴细胞的染色体畸变和细胞致死效应,以检验D_1期细胞遗传学适应性反应是否能导致存活适应性反应.血样来自6名献血员,分离并培养单核细胞层,加PHA 12和18小时后,分别以5cGy(20cGy/min)和200cGy或400cGy(1Gy/min)X射线照射培养物.观察细胞存活和染色体畸变情况.对5人的细胞遗传学分析表明,在5cGy+400cGy组均存在适应性反应,其中3人双着丝粒及环以及2人的缺失显著减少;在5cGy+200cGy组,
The chromosomal aberrations and cell lethal effects of human lymphocytes in the first Gl phase after PHA stimulation with low and high doses of X-rays were analyzed by T-cell cloning to test whether D1 phase cytogenetic adaptive responses could lead to survival. Adaptive response. Blood samples were obtained from 6 blood donors. Monocytes were separated and cultured. After PHA was added for 12 and 18 hours, the cultures were irradiated with 5 cGy (20 cGy/min) and 200 cGy or 400 cGy (1 Gy/min) X-rays, respectively. Observe cell survival and chromosomal aberrations. Cytogenetic analysis of 5 people showed that there was an adaptive response in the 5cGy+400cGy group, in which the loss of dicentrics and rings as well as the loss of 2 in 3 individuals was significantly reduced; in 5cGy+200cGy group,