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探讨ｐ５３和ｒａｓ ｐ２１蛋白的表达与眼睑肿瘤临床预后的关系。方法 采用免疫组化ＳＡＢＣ法检测了９８例眼睑恶性肿瘤组织中ｐ５３和ｒａｓ ｐ２１蛋白的表达。结果 在鳞状细胞癌旁正常及轻度不典型增生上皮中未见ｐ５３和ｒａｓ ｐ２１蛋白表达；而中、重度不典型增生，原位癌，浸润癌ｐ５３和ｒａｓ ｐ２１蛋白阳性表达率逐渐增高。其中基底细胞癌ｐ５３和ｒａｓ ｐ２１阳性表达率分别为５０．０％，５２．５％；鳞状细胞癌分别为５４．３％，６２．９％；睑板腺癌分别为５６．５％，６５．２％。随着肿瘤分化程度的降低，ｐ５３和ｒａｓ ｐ２１蛋白阳性表达率及阳性表达强度逐渐增高；ＳＣＣ中ｐ５３及ｒａｓ ｐ２１蛋白阳性表达与组织分化程度之间呈显著负相关，且与肿瘤淋巴转移有关。结论 ｐ５３和ｒａｓ ｐ２１的表达可作为判断眼睑肿瘤恶性程度、淋巴结转移的重要指标。
To investigate the relationship between p53 and ras p21 protein expression and the clinical prognosis of eyelid tumors. Methods The expressions of p53 and ras p21 protein in 98 cases of malignant eyelid tumors were detected by immunohistochemical SABC method. Results There was no expression of p53 and ras p21 protein in normal and mild dysplastic epithelium adjacent to squamous cell carcinoma. The positive rate of p53 and ras p21 expression in moderate and severe dysplasia, carcinoma in situ, invasive carcinoma p53 and ras p21 protein increased gradually. The positive rates of p53 and ras p21 in basal cell carcinoma were 50.0% and 52.5% respectively, while those in squamous cell carcinoma were 54.3% and 62.9% respectively. The meibomian adenocarcinomas were 56.5% 65.2%. The positive expression rate of p53 and ras p21 protein gradually increased with the decrease of tumor differentiation degree. The positive expression of p53 and ras p21 protein in SCC was negatively correlated with the degree of differentiation, and was related to lymph node metastasis. Conclusion The expression of p53 and ras p21 may be used as an important index for judging the malignancy and lymph node metastasis of eyelid tumors.