论文部分内容阅读
目的检测α-生育酚琥珀酸酯(-αTOS)对MCF7和MDA-MB-453乳腺癌细胞增殖以及细胞中凋亡抑制蛋白家族(IAPs)表达的影响。方法采用四甲基偶氮唑蓝(MTT)法测定-αTOS对人乳腺癌细胞MCF7和MDA-MB-453增殖的抑制作用,细胞分别接种于96孔板后用浓度为51、0、255、0、75、1001、25和150μmol/L的-αTOS处理48 h后检测;细胞经50μmol/L、100μmol/L-αTOS处理122、4、48 h后,用RT-PCR的方法检测细胞内c-IAP1和c-IAP2 mRNA的转录水平;用Western Blot的方法检测c-IAP1和c-IAP2的蛋白质表达水平。结果-αTOS对人乳腺癌细胞MCF7和MDA-MB-453有显著的增殖抑制作用,并表现为剂量依赖性,-αTOS对MCF7和MDA-MB-453的IC50值(细胞半数死亡的药物浓度)分别为132μmol/L和74μmol/L;RT-PCR结果显示,-αTOS使两个细胞系的c-IAP1的转录水平显著下降,且呈时间依赖性;但对c-IAP2的转录水平没有明显影响;Western Blot的结果发现-αTOS使得这两种细胞系的c-IAP1蛋白质翻译水平也呈下降趋势,该结果与mRNA转录水平下降相一致,对c-IAP2蛋白质的表达几乎无影响。因此,α-TOS可能通过抑制c-IAP1蛋白的表达而抑制MCF7和MDA-MB-453乳腺癌细胞的生长增值,a-TOS有望开发成为新的预防和治疗乳腺癌的有效药物。
Objective To investigate the effects of α-tocopherol succinate (-αTOS) on the proliferation of MCF7 and MDA-MB-453 breast cancer cells and the expression of apoptosis-inhibiting protein IAPs. Methods MTT assay was used to determine the inhibitory effect of -αTOS on the proliferation of human breast cancer cell line MCF7 and MDA-MB-453. The cells were seeded into 96-well plates at a concentration of 51,0,255, The cells were treated with 0, 75, 100, 25 and 150μmol / L-αTOS for 48 h. The cells were treated with 50μmol / L, 100μmol / L TOS for 122,4,48 h, - IAP1 and c-IAP2 mRNA transcription levels; Western Blot method was used to detect c-IAP1 and c-IAP2 protein expression levels. Results-αTOS inhibited the proliferation of human breast cancer cells MCF7 and MDA-MB-453 in a dose-dependent manner. The IC50 of ICOS for MCF7 and MDA-MB-453 (drug concentration of half of cells) RT-PCR results showed that -αTOS significantly decreased the c-IAP1 transcription level in both cell lines in a time-dependent manner, but had no significant effect on the transcription level of c-IAP2 ; Western Blot results showed -αTOS make these two cell lines c-IAP1 protein translation also showed a downward trend, the result is consistent with the decline in mRNA transcription level, c-IAP2 protein expression almost no effect. Therefore, α-TOS may inhibit the growth of MCF7 and MDA-MB-453 breast cancer cells by inhibiting the expression of c-IAP1 protein, a-TOS is expected to be developed into a new effective drug for the prevention and treatment of breast cancer.