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本研究探索NQO1C609T,RAD51G135C和XRCC3C241T单核苷酸多态性与急性淋巴细胞白血病(ALL)发生的关系。对170例ALL患者和458名与患者无血缘关系的正常人,用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法分析其NQO1C609T,RAD51G135C和XRCC3C241T基因型。结果表明:在单基因水平分析时NQO1C609T,RAD51G135C和XRCC3C241T基因型比例在正常对照和ALL患者之间无统计学差异,提示其单独作用时对ALL发病的影响无统计学意义。当3个基因联合分析时,NQO1C609T和RAD51G135C均为变异型时伴髓系抗原阳性的ALL和伴平衡易位ALL的发病风险增加(OR值分别为5.553和2.618);NQO1C609T纯合变异型时农村儿童ALL的发病风险增加(OR值为2.541)。结论:NQO1C609T、RAD51G135C和XRCC3C241T基因型联合作用可能促进ALL的发病,提示多基因联合分析较单基因对ALL的发病分析可能更有预测意义。
This study explored the relationship between NQO1C609T, RAD51G135C and XRCC3C241T single nucleotide polymorphisms and the occurrence of acute lymphoblastic leukemia (ALL). The genotypes of NQO1C609T, RAD51G135C and XRCC3C241T were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 170 patients with ALL and 458 healthy persons without relatives. The results showed that there was no significant difference in the proportion of NQO1C609T, RAD51G135C and XRCC3C241T genotypes between normal controls and ALL patients at the level of single gene analysis, suggesting that there was no significant difference in the incidence of ALL when it was administered alone. When combined analysis of three genes, both NQO1C609T and RAD51G135C were associated with an increased risk of myeloid antigen-positive ALL and associated with balanced translocation of ALL (OR 5.553 and 2.618, respectively); NQO1C609T homozygous variant rural The risk of childhood ALL increased (OR = 2.541). Conclusion: The combined effect of NQO1C609T, RAD51G135C and XRCC3C241T genotypes may promote the pathogenesis of ALL, suggesting that multi-gene combination analysis may be more predictive than single gene analysis on the incidence of ALL.