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目的:探讨黄芩苷对氯化钴(CoCl_2)诱导的SHSY-5y细胞损伤的保护作用及其相关作用机制。方法:采用体外培养细胞的方法,建立SHSY-5y细胞CoCl_2损伤模型。分设正常组、CoCl_2损伤组、黄芩苷组(10-8,10-7,10-6,10-5mol/L)。采用MTT法检测细胞活力,光镜观察各组细胞的形态,采用ELISA法检测各组细胞超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)、氧自由基(ROS)的变化。结果:同CoCl_2组比较,黄芩苷能明显减轻CoCl_2引起的SHSY-5y细胞的损伤,降低MDA、ROS的含量,减少LDH的外漏,并升高SOD的活性。结论:黄芩苷对CoCl_2诱导的SHSY-5y细胞损伤具有明显的保护作用,其作用机制可能与黄芩苷抗氧化作用相关,继而减少了细胞的损伤。
Objective: To investigate the protective effect of baicalin on the injury of SHSY-5y cells induced by cobalt chloride (CoCl2) and its related mechanism. Methods: CoSCl-2 injury model was established in SHSY-5y cells cultured in vitro. Divided into normal group, CoCl 2 injury group, baicalin group (10-8,10-7,10-6,10-5mol / L). Cell viability was measured by MTT assay. Morphology of each group was observed with light microscope. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), oxygen free radical (ROS) changes. Results: Compared with CoCl_2 group, baicalin could obviously reduce the injury of SHSY-5y cells induced by CoCl_2, reduce the content of MDA and ROS, reduce the leakage of LDH and increase the activity of SOD. CONCLUSION: Baicalin has a protective effect on CoCl 2 -induced injury of SHSY-5y cells. Its mechanism may be related to the anti-oxidative effect of baicalin, and then reduce the cell damage.