采用单因素和正交试验相结合的方法,对烟色离褶伞(Lyophyllum fumosum)液体菌种培养条件和栽培基质进行优化。结果表明,试验范围内,烟色离褶伞菌种最佳培养条件为:pH值7.5,转速160r/min,250mL三角瓶的装液量150mL,25℃条件下培养11d;最佳出菇栽培基质配方为:30%草炭、28%玉米芯、20%稻壳、10%麦麸、5%豆饼、5%小麦、1%石膏、1%石灰,生物学效率达65%。 The combination of single factor and orthogonal experiment was used to optimize the liquid culture conditions and culture medium of Lyophyllum fumosum. The results showed that the optimum culture conditions of Pleurotus citrinopileatus were as follows: pH value 7.5, speed 160 r / min, volume of 150 mL 250mL flask, culture 11d at 25 ℃; The matrix formula is as follows: 30% peat, 28% corn cob, 20% rice husks, 10% wheat bran, 5% bean cake, 5% wheat, 1% gypsum and 1% lime with biological efficiency of 65%.