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目的 研究阿霉素热化疗对兔鳞癌细胞 (VX- 2 )的毒性作用 .方法 以体外长期培养的兔 VX- 2细胞为靶细胞 ,采用水浴加温法进行体外细胞毒试验 (MTT法 ) ,观察了热疗 ,化疗及热化疗对兔 VX- 2细胞的生长抑制的影响 .结果 温热和阿霉素对 VX- 2均有一定的抑制和杀伤作用 ,一定方式的两者联合具有协同或相加作用 .热化疗加热温度以 42～ 43℃为佳 ,加热时间以 30及 6 0 min为佳 .结论 加热可以明显提高 VX- 2细胞对阿霉素的敏感性
Objective To study the toxic effects of doxorubicin hyperthermia on VX-2 in rabbit.Methods Rabbit VX-2 cells were cultured in vitro and treated by water bath warming method in vitro (MTT method) , The effects of hyperthermia, chemotherapy and thermochemotherapy on the growth inhibition of rabbit VX-2 cells were observed.Results Both warm and doxorubicin inhibited and killed VX-2 in some way and the synergistic effect Or addition.The thermotherapy temperature is preferably 42 ~ 43 ℃, heating time is 30 and 60 min is better.Conclusion Heating can significantly improve the sensitivity of VX-2 cells to doxorubicin