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目的通过流式细胞术测定食管脱落细胞DNA增殖活性指标,以探讨其在食管癌中的诊断价值。方法用食管拉网法获得41例食管癌病人和21例非食管癌病人食管脱落细胞,制成单细胞悬液,作碘化丙啶荧光染色,记录和计算组方图、DNA指数(DI)、G2G1、S期比率(SPF)、细胞增殖活性指数(PI)等指标,同时结合肿瘤的临床病理学特征,并与脱落细胞常规细胞学检查结果进行对比。结果DI:食管癌组1.31±0.23,对照组1.00±0.03;异倍体(An)出现率:食管癌组70.7%,对照组4.8%;G2G1:食管癌组2.071±0.153,对照组1.996±0.099;SPF:食管癌组28.830±15.997,对照组5.804±4.771;PI:食管癌组37.010±13.794,对照组14.738±9.703。两组上述指标的差别均有显著性意义(P<0.05或0.01)。结论食管癌组食管脱落细胞的DI、An以及PI均较对照组明显升高。食管脱落细胞DNA增殖活性指标对食管癌普查及提高早期诊断率具有一定的应用价值。
Objective To determine the index of DNA proliferation activity of esophageal exfoliated cells by flow cytometry to explore its diagnostic value in esophageal cancer. Methods 41 cases of esophageal cancer patients and 21 cases of esophageal cancer patients esophageal exfoliated cells were obtained by esophageal drawing method. Single cell suspension was prepared for fluorescence staining with propidium iodide. The histogram and DNA index (DI) were recorded and calculated. , G2G1, SPF and PI, combined with the clinicopathological characteristics of the tumor, and compared with the conventional cytology results of exfoliated cells. Results DI: 1.31 ± 0.23 in esophageal cancer group and 1.00 ± 0.03 in control group; Occurrence rate of aneuploidy: 70.7% in esophageal cancer group and 4.8% in control group; G2G1: 2.071 ± 0.153 in esophageal cancer group and 1.996 ± 0.099 in control group ; SPF: 28.830 ± 15.997 in esophageal cancer group and 5.804 ± 4.771 in control group; PI: 37.010 ± 13.794 in esophageal cancer group and 14.738 ± 9.703 in control group. The differences between the two groups were significant (P <0.05 or 0.01). Conclusion The esophageal cancer esophageal exfoliated cells DI, An and PI were significantly higher than the control group. Esophageal exfoliated cells DNA proliferation activity of esophageal cancer screening and improve the early diagnosis rate has a certain value.