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目的研究蛋白激酶 C 和 Na~+/H~+交换在表皮生长因子(EGF)促人肝癌细胞生长中的作用.方法采用无血清 RPM11640培养基培养人肝癌细胞SMMC7721,给以 EGF 10~(-9)mol·L~(-1),以~3H-7hymidine(~3H-TdR)掺入法,检测肝癌细胞 DNA 合成的速率.并分别用蛋白激酶 C 阻滞剂 H-7 50μml·L~(-1)和 Na~+/H~+交换阻滞剂amiloride 0.1 mmol·L~(-1)阻断 EGF 对肝癌细胞的促生长作用.结果 EGF 10~(-9)mol·L~(-1)可显著促进肝癌细胞的生长,掺入值为(1880±281)·min~(-1)·孔~(-1),与对照组掺入值(1353±175)·min~(-1).孔~(-1)比较,差异有显著性(P<0.05).单纯 H-7或amiloride,掺入值分别为(1179±150)·min~(-1)·孔~(-1)和(1392±152)·min~(-1)·孔~(-1),与对照组差异无显著性(P>0.05),对肝癌细胞的生长无明显影响;而 H-7,amiloride 与 EGF 合用,掺入值分别为(1241±147)·min~(-1)·孔~(-1)和(1380±189)·min~(-1)·孔~(-1),与 EGF 组相比差异有显著性(P<0.01,P<0.05).结论 EGF 能显著促进肝癌细胞的生长,蛋白激酶 C 和 Na~+/H~+交换在 EGF 的促肝癌细胞生长中起关键作用.
Objective To investigate the role of protein kinase C and Na~+/H~+ exchange in the growth of human hepatocellular carcinoma cells induced by epidermal growth factor (EGF).Methods Human hepatoma cells SMMC7721 were cultured in serum-free RPM11640 medium and given EGF 10~(-). 9)mol·L -1 , 3H-7hymidine (~3H-TdR) incorporation method was used to detect the rate of DNA synthesis of hepatocellular carcinoma cells. Protein kinase C blocker H-7 50μml·L~ was used. (-1) and Na~+/H~+ exchange blocker amiloride 0.1 mmol·L~(-1) blocks the growth-promoting effect of EGF on hepatocellular carcinoma cells. Results EGF 10~(-9) mol·L~( -1) can significantly promote the growth of liver cancer cells, the value of (1880 ± 281) · min ~ (-1) · hole ~ (-1), and the control group dosing value (1353 ± 175) · min ~ ( -1). There was significant difference between the wells (-1) (P<0.05). For the H-7 or amiloride alone, the incorporation values were (1179±150)·min~(-1)·holes~(-). -1) and (1392±152)·min~(-1)·hole~(-1) had no significant difference with the control group (P>0.05), but had no significant effect on the growth of hepatoma cells. ,Amiloride and EGF were used together with the values of (1241±147)·min~(-1)·hole~(-1) and (1380±189)·min~(-1)·hole~(-1) Compared with EGF group, the difference was significant (P<0.01, P<0.05). Conclusions EGF can show In order to promote the growth of hepatocellular carcinoma cells, protein kinase C and Na~+/H~+ exchange play a key role in the growth of hepatoma cells.