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目的:探讨男性睾酮(T)及雄激素结合蛋白(ABP)与饮水氟暴露的关系。方法:应用现况调查研究,在河南省某县随机选择7个村庄作为调查点,分别为高氟村2个、改水村2个和对照村3个;采集各调查点生活饮用水。整群抽取所选调查点本地出生的18~50岁男性作为调查对象,分别采集晨尿和空腹静脉血。应用氟离子选择电极法测定饮用水和尿中氟含量,采用ELISA法测定血清ABP水平,采用化学发光免疫分析法测定血清T。结果:高氟组饮水氟浓度为(2.44±1.88)mg/L,高于对照组的(0.37±0.15)mg/L和改水组的(0.36±0.30)mg/L(F=12.289,P<0.001)。高氟组尿氟浓度为(2.49±1.40)mg/L,高于对照组的(1.04±0.49)mg/L及改水组的(1.38±0.67)mg/L(F=71.563,P<0.001),改水组亦高于对照组(P<0.05)。高氟组血清ABP含量为(16.01±10.83)nmol/L,低于对照组的(27.94±31.90)nmol/L及改水组的(22.42±28.12)nmol/L(F=28.807,P<0.001)。对照组、改水组、高氟组血清T含量为(4.31±1.30)、(4.42±1.37)和(4.74±2.17)nmol/L,差异无统计学意义(F=0.268,P=0.765)。在对照组和改水组中,血清T含量与年龄呈负相关(β=-0.238、-0.262,P均<0.05)。结论:环境氟暴露可影响男性血清ABP水平。
Objective: To investigate the relationship between male testosterone (T) and androgen binding protein (ABP) and fluoride exposure in drinking water. Methods: According to the current situation of investigation and study, seven villages were selected at random in a county in Henan Province as survey sites, including 2 high fluoride village, 2 water improvement villages and 3 control villages; and collected drinking water from various survey sites. A total of 18-5 old men born locally from the selected survey sites were enrolled in the study. Morning urine and fasting blood were collected respectively. Fluorine ion selective electrode method was used to determine the content of fluoride in drinking water and urine. The level of serum ABP was measured by ELISA. The level of serum T was measured by chemiluminescence immunoassay. Results: The fluorine level in high fluoride group was (2.44 ± 1.88) mg / L, higher than that in control group (0.37 ± 0.15) mg / L and water group (0.36 ± 0.30) mg / L <0.001). The urinary fluoride concentration in high fluoride group was (2.49 ± 1.40) mg / L, higher than that in control group (1.04 ± 0.49) mg / L and (1.38 ± 0.67) mg / L ), Water change group is also higher than the control group (P <0.05). The serum level of ABP in high fluoride group was (16.01 ± 10.83) nmol / L, lower than that in control group (27.94 ± 31.90) nmol / L and (22.42 ± 28.12) nmol / L ). The serum levels of T in control group, water modification group and high fluoride group were (4.31 ± 1.30), (4.42 ± 1.37) and (4.74 ± 2.17) nmol / L, respectively. There was no significant difference between the two groups (F = 0.268, P = 0.765). In control group and water modification group, serum T content was negatively correlated with age (β = -0.238, -0.262, P <0.05). Conclusion: Environmental fluoride exposure can affect male serum ABP levels.