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目的：通过对合肥地区血透患者及献血员丙肝病毒感者ＨＣＶ基因分布的调查，了解合肥地区ＨＣＶ基因型分布，从而为丙型病毒性肝炎的防治提供依据。方法：采用异硫氰酸胍一步法提取核酸，将ＰＣＲ产物在２％琼脂糖凝胶中电泳，溴乙碇染色，置紫外灯下判断结果。结果：８４例抗－ＨＣＶ阳性者，ＨＣＶＲＮＡ阳性者为６１例，６１例中ＨＣＶ－Ⅱ６０例占９８．３６ ％，ＨＣＶ－Ⅲ只有１例，占１．６４ ％。结论：表明合肥地区ＨＣＶ 感染以Ⅰｂ／Ⅱ占绝大多数，未见混合感染和其他基因型。
OBJECTIVE: To investigate the distribution of HCV genotypes in hemodialysis patients and donors of hepatitis C virus infection in Hefei area to understand the distribution of HCV genotypes in Hefei, and to provide evidence for the prevention and treatment of hepatitis C virus. Methods: Nucleic acid was extracted by one-step method with guanidine isothiocyanate. The PCR products were electrophoresed in 2% agarose gel and stained with ethidium bromide. The results were judged under UV light. Results: Of the 84 anti-HCV positive cases, 61 cases were HCV RNA positive, 60 cases HCV-Ⅱ60 cases accounted for 98.36%, and HCV-Ⅲ only 1 case, accounting for 1.64%. Conclusion: The prevalence of HCV infection in Hefei is in the majority of Ⅰb / Ⅱ, no mixed infection and other genotypes are found.