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目的探讨FFA增加肝脏葡萄糖生成和肝脏胰岛素抵抗(IR)的作用位点。方法分别给清醒大鼠输注24h脂肪乳+肝素和盐水,在输注的最后2h行高胰岛素正葡萄糖钳夹试验,测定肝脏葡萄糖生成率(HGP)、总葡萄糖输出率(TGO)和葡萄糖循环(GC)。结果脂肪乳使血浆FFA升高3倍。在基础状态,脂肪乳使HGP和TGO增高(P<0.01)。在钳夹状态,脂肪乳降低了胰岛素(Ins)增加GC的作用(P<0.01),并使Ins抑制HGP和TGO的作用下降(P<0.01)。结论 FFA能使Ins增加GC和抑制HGP与TGO的作用下降,说明FFA在不同位点诱导了肝脏IR。
Objective To investigate the role of FFA in increasing hepatic glucose production and hepatic insulin resistance (IR). Methods 24 h fat milk, heparin and saline were respectively transfused into awake rats, and hyperinsulinemic positive clamp was performed in the last 2 hours of infusion. The hepatic glucose production (HGP), total glucose output (TGO) and glucose cycle (GC). Results Fatty milk increased plasma FFA by a factor of 3. In the basal state, fat emulsion increased HGP and TGO (P <0.01). In the clamped state, the fat emulsion decreased the effect of insulin on GC (P <0.01) and decreased the effect of Ins on HGP and TGO (P <0.01). Conclusion FFA can make Ins increase GC and inhibit the decrease of HGP and TGO, indicating that FFA induces hepatic IR at different sites.